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A neuroprotective role for angiogenin in models of Parkinson’s disease
Author(s) -
Steidinger Trent U.,
Standaert David G.,
Yacoubian Talene A.
Publication year - 2011
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2010.07112.x
Subject(s) - angiogenin , neuroprotection , programmed cell death , amyotrophic lateral sclerosis , neuroblastoma , protein kinase b , neurodegeneration , mptp , sh sy5y , phosphorylation , glial cell line derived neurotrophic factor , microbiology and biotechnology , chemistry , angiogenesis , biology , cancer research , neurotrophic factors , pharmacology , apoptosis , medicine , cell culture , dopamine , dopaminergic , biochemistry , neuroscience , receptor , disease , genetics
J. Neurochem. (2011) 116 , 334–341. Abstract We previously observed marked down‐regulation of the mRNA for angiogenin, a potent inducer of neovascularization, in a mouse model of Parkinson’s disease (PD) based on over‐expression of alpha‐synuclein. Angiogenin has also been recently implicated in the pathogenesis of amyotrophic lateral sclerosis. In this study, we confirmed that mouse angiogenin‐1 protein is dramatically reduced in this transgenic alpha‐synuclein mouse model of PD, and examined the effect of angiogenin in cellular models of PD. We found that endogenous angiogenin is present in two dopamine‐producing neuroblastoma cell lines, SH‐SY5Y and M17, and that exogenous angiogenin is taken up by these cells and leads to phosphorylation of Akt. Applied angiogenin protects against the cell death induced by the neurotoxins 1‐methyl‐4‐phenylpyridinium and rotenone and reduces the activation of caspase 3. Together our data supports the importance of angiogenin in protecting against dopaminergic neuronal cell death and suggests its potential as a therapy for PD.