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Synthesis, accumulation, and release of d ‐aspartate in the Aplysia californica CNS
Author(s) -
Scanlan Cory,
Shi Ting,
Hatcher Nathan G.,
Rubakhin Stanislav S.,
Sweedler Jonathan V.
Publication year - 2010
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2010.07020.x
Subject(s) - aplysia , ionomycin , extracellular , biochemistry , neuron , biology , endogeny , microbiology and biotechnology , chemistry , biophysics , neuroscience , in vitro
J. Neurochem. (2010) 115 , 1234–1244. Abstract d ‐Aspartate ( d ‐Asp) is an endogenous molecule that is often detected in CNS and endocrine tissues. Using capillary electrophoresis and a variety of radionuclide detection techniques, we examine the synthesis, release, and uptake/accumulation of d ‐Asp in the CNS of the marine mollusk Aplysia californica . We observe the preferential synthesis and accumulation of d ‐Asp over l ‐aspartate ( l ‐Asp) in neuron‐containing ganglia compared to surrounding sheath tissues. Little conversion of d ‐Asp to l ‐Asp is detected. The Ca 2+ ionophore ionomycin and elevated extracellular potassium stimulates release of d ‐Asp from the cerebral ganglia. Lastly, radioactive d ‐Asp in the extracellular media is efficiently taken up and accumulated by individual F‐cluster neurons. These observations point to a role for d ‐Asp in cell‐to‐cell signaling with many characteristics similar to classical transmitters.