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Chromogranins as regulators of exocytosis
Author(s) -
Borges Ricardo,
DíazVera Jésica,
Domínguez Natalia,
Arnau María Rosa,
Machado José D.
Publication year - 2010
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2010.06786.x
Subject(s) - exocytosis , vesicle , secretory vesicle , amperometry , microbiology and biotechnology , chromaffin cell , secretion , neurotransmitter , chemistry , extracellular , biochemistry , vesicle fusion , intracellular , synaptic vesicle , biology , adrenal medulla , catecholamine , membrane , receptor , neuroscience , electrode , electrochemistry
J. Neurochem. (2010) 114 , 335–343. Abstract Chromogranins (Cgs) constitute the main protein component in the vesicular matrix of large dense core vesicles (LDCV). These acidic proteins have been implicated in several physiological processes such as vesicle sorting, the generation of bioactive peptides and the accumulation of soluble species inside LDCV. This latter feature of Cgs accounts for the ability of vesicles to concentrate catecholamines and Ca 2+ . Indeed, the low affinity and high capacity of Cgs to bind solutes at the low pH of the LDCV lumen seems to be behind the delay in the neurotransmitter exit towards the extracellular milieu after vesicle fusion. The availability of new mouse strains lacking Cgs in combination with the arrival of several techniques for the direct monitoring of exocytosis (like amperometry, patch‐amperometry and intracellular electrochemistry), have helped advance our understanding of how these granins concentrate catecholamines and Ca 2+ in LDCV, and how they influence the kinetics of exocytosis. In this review, we will discuss the roles of Cgs A and B in maintaining the intravesicular environment of secretory vesicles and in exocytosis, bringing together the most recent findings from adrenal chromaffin cells.