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Temporal dysregulation of cortical gene expression in the isolation reared Wistar rat
Author(s) -
Murphy Keith J.,
Ter Horst Judith P. F.,
Cassidy Andrew W.,
DeSouza Ian E. J.,
Morgunova Marina,
Li Christine,
Connole Laura M.,
O’Sullivan Niamh C.,
Loscher Jennifer S.,
Brady Angela T.,
Rombach Nanette,
Connellan Joanna,
McGettigan Paul A.,
Scully Darren,
Fedriani Rocio,
Lukasz Bartlomiej,
Moran Mary P.,
McCabe Olive M.,
Wantuch Caitlin M.,
Hughes Zoe A.,
Mulvany Sean K.,
Higgins Desmond G.,
Pangalos Menelas N.,
Marquis Karen L.,
O’Connor William T.,
Ring Robert H.,
Von Schack David,
Regan Ciaran M.
Publication year - 2010
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2010.06617.x
Subject(s) - neurochemical , neuroscience , prefrontal cortex , biology , synapse , neurotransmission , hippocampus , glutamatergic , glutamate receptor , genetics , receptor , cognition
J. Neurochem. (2010) 113 , 601–614. Abstract The critical sequence of molecular, neurotransmission and synaptic disruptions that underpin the emergence of psychiatric disorders like schizophrenia remain to be established with progress only likely using animal models that capture key features of such disorders. We have related the emergence of behavioural, neurochemical and synapse ultrastructure deficits to transcriptional dysregulation in the medial prefrontal cortex of Wistar rats reared in isolation. Isolation reared animals developed sensorimotor deficits at postnatal day 60 which persisted into adulthood. Analysis of gene expression prior to the emergence of the sensorimotor deficits revealed a significant disruption in transcriptional control, notably of immediate early and interferon‐associated genes. At postnatal day 60 many gene transcripts relating particularly to GABA transmission and synapse structure, for example Gabra4, Nsf, Syn2 and Dlgh1, transiently increased expression. A subsequent decrease in genes such as Gria2 and Dlgh2 at postnatal day 80 suggested deficits in glutamatergic transmission and synapse integrity, respectively. Microdialysis studies revealed decreased extracellular glutamate suggesting a state of hypofrontality while ultrastructural analysis showed total and perforated synapse complement in layer III to be significantly reduced in the prefrontal cortex of postnatal day 80 isolated animals. These studies provide a molecular framework to understand the developmental emergence of the structural and behavioural characteristics that may in part define psychiatric illness.