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Phosphoinositide 3‐kinase mediated signaling in lobster olfactory receptor neurons
Author(s) -
Corey Elizabeth A.,
Bobkov Yuriy,
Pezier Adeline,
Ache Barry W.
Publication year - 2010
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2010.06597.x
Subject(s) - olfactory receptor , biology , signal transduction , microbiology and biotechnology , olfactory system , neuroscience
J. Neurochem. (2010) 113 , 341–350. Abstract In vertebrates and some invertebrates, odorant molecules bind to G protein‐coupled receptors on olfactory receptor neurons (ORNs) to initiate signal transduction. Phosphoinositide 3‐kinase (PI3K) activity has been implicated physiologically in olfactory signal transduction, suggesting a potential role for a G protein‐coupled receptor‐activated class I PI3K. Using isoform‐specific antibodies, we identified a protein in the olfactory signal transduction compartment of lobster ORNs that is antigenically similar to mammalian PI3Kγ and cloned a gene for a PI3K with amino acid homology with PI3Kβ. The lobster olfactory PI3K co‐immunoprecipitates with the G protein α and β subunits, and an odorant‐evoked increase in phosphatidylinositol (3,4,5)‐trisphosphate can be detected in the signal transduction compartment of the ORNs. PI3Kγ and β isoform‐specific inhibitors reduce the odorant‐evoked output of lobster ORNs in vivo . Collectively, these findings provide evidence that PI3K is indeed activated by odorant receptors in lobster ORNs and further support the potential involvement of G protein activated PI3K signaling in olfactory transduction.