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CREB‐dependent Nur77 induction following depolarization in PC12 cells and neurons is modulated by MEF2 transcription factors
Author(s) -
Lam Brian Yee Hong,
Zhang Wenting,
Ng David ChiHeng,
Maruthappu Mahiben,
Roderick Hywel Llewelyn,
Chawla Sangeeta
Publication year - 2010
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2009.06521.x
Subject(s) - nerve growth factor ib , creb , mef2 , transcription factor , enhancer , biology , microbiology and biotechnology , depolarization , repressor , nuclear receptor , gene , endocrinology , genetics
J. Neurochem . (2010) 112 , 1065–1073. Abstract Expression of the nuclear orphan receptor gene Nur77 in neuronal cells is induced by activity‐dependent increases in intracellular Ca 2+ ions. Ca 2+ responsiveness of the Nur77 gene has been attributed to two distinct DNA regulatory regions that recruit the transcription factors cAMP response element binding protein (CREB) and myocyte enhancer factor‐2 (MEF2). Here we used dominant interfering and constitutively active mutants of CREB and MEF2 proteins to assess their relative contribution to depolarization‐induced Nur77 expression in undifferentiated PC12 cells and hippocampal neurons. We show that while CREB is necessary for Ca 2+ ‐activated Nur77 expression MEF2 functions to modulate CREB‐dependent Nur77 expression by acting as a repressor in quiescent cells.