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OBCAM, an immunoglobulin superfamily cell adhesion molecule, regulates morphology and proliferation of cerebral astrocytes
Author(s) -
Sugimoto Chiaki,
Maekawa Shohei,
Miyata Seiji
Publication year - 2010
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2009.06513.x
Subject(s) - immunoglobulin superfamily , microbiology and biotechnology , cell growth , biology , astrocyte , cell adhesion molecule , cell adhesion , in vivo , cell type , neural cell adhesion molecule , cell , chemistry , biochemistry , neuroscience , central nervous system , genetics
J. Neurochem. (2010) 112 , 830–839. Abstract Opioid‐binding cell adhesion molecule (OBCAM) is a member of the immunoglobulin superfamily. In the present study, we investigated the expression and functional significance of OBCAM in cerebral astrocytes in vitro and in vivo . The confocal microscopic study revealed that the expression of OBCAM was prominent at filopodia and cellular processes of type‐1 and ‐2 astrocytes. Moreover, the expression of OBCAM was shown to be stronger at the growth phase than the stationary one in type‐1 ones. Ki‐67‐positive proliferating type‐1 astrocytes showed higher frequency of OBCAM expression as compared with Ki‐67‐negative cells. The application of the anti‐OBCAM IgG facilitated the proliferation of type‐1 astrocytes at relatively low concentration (1 and 5 μg/mL) and had no effects at relatively high concentration (25 and 50 μg/mL). The cellular proliferation was not promoted when the anti‐OBCAM Fab fragment was used, indicating that the antibody‐induced clustering of OBCAM promotes the proliferation. The over‐expression of OBCAM increased the cell size of type‐1 astrocytes. Moreover, intense expression of OBCAM was observed on reactive astrocytes in the injured cerebrum in vivo . These results suggest that OBCAM is responsible for controlling the cell proliferation and growth in cortical astrocytes.