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Disruption of the interaction between myosin VI and SAP97 is associated with a reduction in the number of AMPARs at hippocampal synapses
Author(s) -
Nash Joanne E.,
Appleby Vanessa J.,
Corrêa Sonia A. L.,
Wu Hongju,
Fitzjohn Stephen M.,
Garner Craig C.,
Collingridge Graham L.,
Molnár Elek
Publication year - 2010
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2009.06480.x
Subject(s) - ampa receptor , postsynaptic potential , excitatory postsynaptic potential , synapse , silent synapse , postsynaptic density , microbiology and biotechnology , myosin , chemistry , hippocampal formation , glutamate receptor , neuroscience , biology , receptor , biophysics , biochemistry
J. Neurochem. (2010) 112 , 677–690. Abstract Myosin VI is an actin‐based motor protein that is enriched at the postsynaptic density and appears to interact with alpha‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole propionate‐type glutamate receptors (AMPARs) via synapse associated protein 97 (SAP97). Here, we find that a Flag epitope‐tagged dominant negative construct that inhibits the interaction between SAP97 and myosin VI (Flag‐myoVI‐DN) causes a dramatic reduction in the number of synapses and the surface expression of AMPARs in cultured hippocampal neurons. Furthermore, we find that Flag‐myoVI‐DN also prevents the rapid delivery of AMPARs to synapses that can be induced by the transient activation of N ‐methyl‐ d ‐aspartate receptors. The Flag‐myoVI‐DN induced decrease in surface AMPARs is not because of reduced AMPAR subunit protein synthesis. Using whole‐cell recording, we show that Flag‐myoVI‐DN also prevents the activity‐induced increase in miniature excitatory postsynaptic current frequency that is normally associated with recruitment of AMPARs to the cell surface at synaptic sites that lack these receptors (i.e. ‘silent’ synapses). Together, these results indicate that myosin VI/SAP97 plays an important role in trafficking and activity‐dependent recruitment of AMPARs to synapses.