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In vivo neurochemical profiling of rat brain by 1 H‐[ 13 C] NMR spectroscopy: cerebral energetics and glutamatergic/GABAergic neurotransmission
Author(s) -
Van Eijsden Pieter,
Behar Kevin L.,
Mason Graeme F.,
Braun Kees P. J.,
De Graaf Robin A.
Publication year - 2010
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2009.06428.x
Subject(s) - glutamatergic , neurotransmission , gabaergic , neurochemical , in vivo , glutamate receptor , neurotransmitter , neuroscience , excitatory postsynaptic potential , nuclear magnetic resonance spectroscopy , chemistry , spectroscopy , in vivo magnetic resonance spectroscopy , gamma aminobutyric acid , nuclear magnetic resonance , biology , inhibitory postsynaptic potential , biochemistry , medicine , central nervous system , magnetic resonance imaging , stereochemistry , physics , receptor , microbiology and biotechnology , quantum mechanics , radiology
J. Neurochem. (2010) 112 , 24–33. Abstract The quantification of excitatory and inhibitory neurotransmission and the associated energy metabolism is crucial for a proper understanding of brain function. Although the detection of glutamatergic neurotransmission in vivo by 13 C NMR spectroscopy is now relatively routine, the detection of GABAergic neurotransmission in vivo has remained elusive because of the low GABA concentration and spectral overlap. Using 1 H‐[ 13 C] NMR spectroscopy at high magnetic field in combination with robust spectral modeling and the use of different substrates, [U‐ 13 C 6 ]‐glucose and [2‐ 13 C]‐acetate, it is shown that GABAergic, as well as glutamatergic neurotransmitter fluxes can be detected non‐invasively in rat brain in vivo .