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CRMP‐2 directly binds to cytoplasmic dynein and interferes with its activity
Author(s) -
Arimura Nariko,
Hattori Atsushi,
Kimura Toshihide,
Nakamuta Shinichi,
Funahashi Yasuhiro,
Hirotsune Shinji,
Furuta Kenya,
Urano Takashi,
Toyoshima Yoko Y.,
Kaibuchi Kozo
Publication year - 2009
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2009.06317.x
Subject(s) - dynein , microtubule , kinesin , microbiology and biotechnology , dynactin , motor protein , axoplasmic transport , axon , biology , cytoplasm , molecular motor , organelle , chemistry
The active transport of proteins and organelles is critical for cellular organization and function in eukaryotic cells. A substantial portion of long‐distance transport depends on the opposite polarity of the kinesin and dynein family molecular motors to move cargo along microtubules. It is increasingly clear that many cargo molecules are moved bi‐directionally by both sets of motors; however, the regulatory mechanism that determines the directionality of transport remains unclear. We previously reported that collapsin response mediator protein‐2 (CRMP‐2) played key roles in axon elongation and neuronal polarization. CRMP‐2 was also found to associate with the anterograde motor protein Kinesin‐1 and was transported with other cargoes toward the axon terminal. In this study, we investigated the association of CRMP‐2 with a retrograde motor protein, cytoplasmic dynein. Immunoprecipitation assays showed that CRMP‐2 interacted with cytoplasmic dynein heavy chain. Dynein heavy chain directly bound to the N‐terminus of CRMP‐2, which is the distinct side of CRMP‐2’s kinesin light chain‐binding region. Furthermore, over‐expression of the dynein‐binding fragments of CRMP‐2 prevented dynein‐driven microtubule transport in COS‐7 cells. Given that CRMP‐2 is a key regulator of axon elongation, this interference with cytoplasmic dynein function by CRMP‐2 might have an important role in axon formation, and neuronal development.