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Agonist‐induced internalization of mGluR1α is mediated by caveolin
Author(s) -
Hong Yun Hwa,
Kim Ji Young,
Lee Jeong Ho,
Chae Hong Gu,
Jang Sung Soo,
Jeon Ju Hong,
Kim Chul Hoon,
Kim Jun,
Kim Sang Jeong
Publication year - 2009
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2009.06289.x
Subject(s) - internalization , metabotropic glutamate receptor 1 , metabotropic glutamate receptor , agonist , metabotropic receptor , microbiology and biotechnology , dynamin , caveolae , clathrin , biology , chemistry , caveolin , endocytosis , signal transduction , neuroscience , receptor , biochemistry
Agonist‐induced internalization of metabotropic glutamate receptors (mGluRs) plays an important role in neuronal signaling. Although internalization of mGluRs has been reported to be mediated by clathrin‐dependent pathway, studies describing clathrin‐independent pathways are emerging. Here, we report that agonist‐induced internalization of mGluR1α is mediated by caveolin. We show that two caveolin‐binding motifs of mGluR1α interact with caveolin1/2. Using cell surface‐immunoprecipitation and total internal reflection fluorescence imaging, we found that agonist‐induced internalization of mGluR1α is regulated by caveolin‐binding motifs of the receptor in heterologous cells. Moreover, in the cerebellum, group I mGluR agonist dihydroxyphenylglycol increased the interaction of phosphorylated caveolin with mGluR1α. This interaction was blocked by methyl‐β‐cyclodextrin, known to disrupt caveolin/caveolae‐dependent signaling by cholesterol depletion. Methyl‐β‐cyclodextrin also blocked the agonist‐induced internalization of mGluR1α. Thus, these findings represent the evidence for agonist‐induced internalization of mGluR1α via caveolin and suggest that caveolin might play a role in synaptic metaplasticity by regulating internalization of mGluR1α in the cerebellum.