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Cyclic AMP‐mediated long‐term facilitation of glycinergic transmission in developing spinal dorsal horn neurons
Author(s) -
Choi InSun,
Nakamura Michiko,
Cho JinHwa,
Park HyeMi,
Kim SangJung,
Kim Jun,
Lee JongJu,
Choi ByungJu,
Jang IlSung
Publication year - 2009
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2009.06275.x
Subject(s) - forskolin , glycine receptor , neuroscience , neurotransmission , inhibitory postsynaptic potential , biology , postsynaptic potential , adenylyl cyclase , microbiology and biotechnology , chemistry , stimulation , receptor , biochemistry , glycine , amino acid
cAMP is known to regulate neurotransmitter release via protein kinase A (PKA)‐dependent and/or PKA‐independent signal transduction pathways at a variety of central synapses. Here we report the cAMP‐mediated long‐lasting enhancement of glycinergic transmission in developing rat spinal substantia gelatinosa neurons. Forskolin, an adenylyl cyclase activator, elicited a long‐lasting increase in the amplitude of nerve‐evoked glycinergic inhibitory postsynaptic currents (IPSCs), accompanied by a long‐lasting decrease in the paired‐pulse ratio in immature substantia gelatinosa neurons, and this forskolin‐induced increase in glycinergic IPSCs decreased with postnatal development. Forskolin also decreased the failure rate of glycinergic IPSCs evoked by minimal stimulation, and increased the frequency of glycinergic miniature IPSCs. All of these data suggest that forskolin induces the long‐lasting enhancement of glycinergic transmission by increasing in the presynaptic release probability. This pre‐synaptic action of forskolin was mediated by hyperpolarization and cyclic nucleotide‐activated cation channels and an increase in intraterminal Ca 2+ concentration but independent of PKA. The present results suggest that cAMP‐dependent signal transduction pathways represent a dynamic mechanism by which glycinergic IPSCs could potentially be modulated during postnatal development.