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A highly conserved tryptophan residue in the fourth transmembrane domain of the A 1 adenosine receptor is essential for ligand binding but not receptor homodimerization
Author(s) -
Suzuki Tokiko,
Namba Kazunori,
Yamagishi Ryosuke,
Kaneko Hiroki,
Haga Tatsuya,
Nakata Hiroyasu
Publication year - 2009
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2009.06227.x
Subject(s) - g protein coupled receptor , transmembrane domain , rhodopsin , adenosine receptor , receptor , biochemistry , förster resonance energy transfer , biophysics , mutant , binding site , biology , ligand (biochemistry) , chemistry , microbiology and biotechnology , agonist , gene , fluorescence , retinal , physics , quantum mechanics
Dimerization between G protein‐coupled receptors (GPCRs) is a clearly established phenomenon. However, limited information is currently available on the interface essential for this process. Based on structural comparisons and sequence homology between rhodopsin and A 1 adenosine receptor (A 1 R), we initially hypothesized that four residues in transmembrane (TM) 4 and TM5 are involved in A 1 R homodimerization. Accordingly, these residues were substituted with Ala by site‐directed mutagenesis. Interestingly, the mutant protein displayed no significant decrease in homodimer formation compared with wild‐type A 1 R, as evident from coimmunoprecipitation and BRET 2 analyses (improved bioluminescence resonance energy transfer system offered by Perkin‐Elmer Life Sciences), but lost ligand binding activity almost completely. Further studies disclosed that this effect was derived from the mutation of one particular residue, Trp132, which is highly conserved among many GPCRs. Confocal immunofluorescence and cell‐surface biotinylation studies revealed that the mutant receptors localized normally at transfected cell membranes, signifying that loss of ligand binding was not because of defective cellular trafficking. Molecular modeling of the A 1 R‐ligand complex disclosed that Trp132 interacted with several residues located in TM3 and TM5 that stabilized agonist binding. Thus, loss of interactions of Trp with these residues may, in turn, disrupt binding to agonists. Our study provides strong evidence of the essential role of the highly conserved Trp132 in TM4 of adenosine receptors.