Premium
The brain selenoproteome: priorities in the hierarchy and different levels of selenium homeostasis in the brain of selenium‐deficient rats
Author(s) -
Kühbacher Markus,
Bartel Jürgen,
Hoppe Barbara,
Alber Dorothea,
Bukalis Gregor,
Bräuer Anja U.,
Behne Dietrich,
Kyriakopoulos Antonios
Publication year - 2009
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2009.06109.x
Subject(s) - selenium , homeostasis , chemistry , in vivo , biochemistry , radiochemistry , biology , endocrinology , microbiology and biotechnology , organic chemistry
The application of radionuclides for the localization of essential trace elements in vivo and the characterization of their binding proteins is a story of intermittently made improvements of the techniques used for their detection. In this study we present the use of neutron activation analysis and different autoradiographic imaging methods including real‐time digital autoradiography to reveal new insights in the hierarchy of selenium homeostasis. Selenoproteins containing the essential trace element selenium play important roles in the CNS. Although the CNS does not show the highest selenium concentration in the case of selenium‐sufficient supply in comparison with other organs, it shows a high priority for selenium uptake and retention in the case of dietary selenium deficiency. To characterize the hierarchy of selenium supply in the brain, in vivo radiotracer labeling with 75 Se in rats with different selenium status was combined with autoradiographic detection of 75 Se in brain tissue sections and 75 Se‐labeled selenoproteins after protein separation by two‐dimensional gel electrophoresis. This study demonstrates significant differences in the uptake of 75 Se into the brain of rats with different selenium status. A brain region‐specific uptake pattern of the radiotracer 75 Se in selenium‐deficient rats could be revealed and the CSF was identified as a key part of the brain selenium homeostasis.