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The interplay between inorganic phosphate and amino acids determines zinc solubility in brain slices
Author(s) -
Rumschik Sean M.,
Nydegger Irma,
Zhao Jinfu,
Kay Alan R.
Publication year - 2009
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2009.05880.x
Subject(s) - zinc , extracellular , amino acid , phosphate , solubility , histidine , zinc phosphate , precipitation , chemistry , inorganic phosphate , biochemistry , molar concentration , aqueous solution , inorganic chemistry , biophysics , biology , organic chemistry , physics , meteorology
Inorganic phosphate (Pi) is an important polyanion needed for ATP synthesis and bone formation. As it is found at millimolar levels in plasma, it is usually incorporated as a constituent of artificial CSF formulations for maintaining brain slices. In this paper, we show that Pi limits the extracellular zinc concentration by inducing metal precipitation. We present data suggesting that amino acids like histidine may counteract the Pi‐induced zinc precipitation by the formation of soluble zinc complexes. We propose that the interplay between Pi and amino acids in the extracellular space may influence the availability of metals for cellular uptake.