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Preconditioning induces prolonged expression of transcription factors pCREB and NF‐κB in the neocortex of rats before and following severe hypobaric hypoxia
Author(s) -
Rybnikova Elena,
Gluschenko Tatjana,
Tulkova Ekaterina,
Churilova Anna,
Jaroshevich Oksana,
Baranova Ksenia,
Samoilov Michail
Publication year - 2008
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2008.05516.x
Subject(s) - creb , neocortex , hypoxia (environmental) , neuroprotection , endocrinology , medicine , transcription factor , biology , chemistry , neuroscience , biochemistry , gene , organic chemistry , oxygen
Preconditioning using mild repetitive hypobaric hypoxia is known to increase a tolerance of brain neurons to severe hypoxia and other injurious exposures. In the present study, the effects of mild hypoxic preconditioning on the expression of transcription factors NF‐κB and phosphorylated CREB (pCREB) has been studied in the neocortex of rats exposed to severe hypobaric hypoxia. As revealed by quantitative immunocytochemistry, the injurious severe hypobaric hypoxia (180 Torr, 3 h) remarkably reduced the neocortical levels of pCREB and NF‐κB. The three‐trial hypoxic preconditioning (360 Torr, 2 h, 3 days) induced persistent up‐regulation of pCREB and NF‐κB expression in the neocortex of rats 3–24 h following the severe hypoxia. In addition, the preconditioning alone which was not followed by the severe hypoxia, considerably increased neocortical pCREB and NF‐κB levels. The findings suggest a role for transcription factors cAMP response element‐binding protein and NF‐κB in the neuroprotective mechanisms activated by the hypoxic preconditioning.