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Local stimulation of the adenosine A 2B receptors induces an increased release of IL‐6 in mouse striatum: an in vivo microdialysis study
Author(s) -
Vazquez Juan Francisco,
Clement HansWilli,
Sommer Olaf,
Schulz Eberhard,
Van Calker Dietrich
Publication year - 2008
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2007.05191.x
Subject(s) - microdialysis , adenosine , adenosine receptor , adenosine a1 receptor , agonist , receptor , stimulation , adenosine a2b receptor , medicine , receptor antagonist , in vivo , adenosine a3 receptor , endocrinology , striatum , chemistry , purinergic signalling , pharmacology , adenosine receptor antagonist , biology , antagonist , central nervous system , dopamine , microbiology and biotechnology
Both adenosine and interleukin‐6 (IL‐6) have been implicated in the pathophysiology of, e.g., epileptic seizures, traumatic brain injury, and affective disorders. Stimulation of adenosine A 2B receptors on astrocytes in vitro leads to the increased synthesis and secretion of IL‐6. We investigated whether or not activation of adenosine receptors evokes an increase of IL‐6 release also in vivo . 5′‐ N ‐ethylcarboxamidoadenosine, a non‐specific adenosine‐agonist or vehicle was administered into the striatum of freely moving mice by reverse microdialysis. A statistical significant increase of the IL‐6 concentration in the perfusate was detected already 60 min after 5′‐ N ‐ethylcarboxamidoadenosine administration. IL‐6 increased progressively and reached a maximum after 240 min. This effect appears to be mediated through adenosine A 2B receptors since it was counteracted by the specific A 2B receptor antagonist MRS1706 but not by the specific A 1 receptor antagonist DPCPX. We conclude that adenosine via activation of A 2B receptors evokes IL‐6 release also in vivo .