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Characterization of mature rat oligodendrocytes: a proteomic approach
Author(s) -
Dumont Debora,
Noben JeanPaul,
Moreels Marjan,
Vanderlocht Joris,
Hellings Niels,
Vandenabeele Frank,
Lambrichts Ivo,
Stinissen Piet,
Robben Johan
Publication year - 2007
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2007.04575.x
Subject(s) - oligodendrocyte , myelin , proteomics , remyelination , biology , immunocytochemistry , gel electrophoresis , microbiology and biotechnology , central nervous system , neuroglia , two dimensional gel electrophoresis , proteome , biochemistry , neuroscience , gene , endocrinology
Oligodendrocytes are glial cells responsible for the synthesis and maintenance of myelin in the central nervous system (CNS). Oligodendrocytes are vulnerable to damage occurring in a variety of neurological diseases. Understanding oligodendrocyte biology is crucial for the dissemination of de‐ and remyelination mechanisms. The goal of the present study is the construction of a protein database of mature rat oligodendrocytes. Post‐mitotic oligodendrocytes were isolated from mature Wistar rats and subjected to immunocytochemistry. Proteins were extracted and analyzed by means of two‐dimensional gel electrophoresis and two‐dimensional liquid chromatography, both coupled to mass spectrometry. The combination of the gel‐based and gel‐free approach resulted in confident identification of a total of 200 proteins. A minority of proteins were identified in both proteomic strategies. The identified proteins represent a variety of functional groups, including novel oligodendrocyte proteins. The results of this study emphasize the power of the applied proteomic strategy to study known or to reveal new proteins and to investigate their regulation in oligodendrocytes in different disease models.