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Mass spectrometric characterization and physiological actions of VPNDWAHFRGSWamide, a novel B type allatostatin in the crab, Cancer borealis
Author(s) -
Fu Qiang,
Tang Lamont S.,
Marder Eve,
Li Lingjun
Publication year - 2007
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2007.04482.x
Subject(s) - stomatogastric ganglion , neuropil , neuromodulation , ganglion , biology , medicine , chemistry , endocrinology , neuroscience , rhythm , central nervous system , central pattern generator
Abstract The neural networks in the crustacean stomatogastric ganglion are modulated by neuroactive substances released locally into the neuropil of the stomatogastric ganglion and by circulating hormones released by neuroendocrine structures including the pericardial organs. Using nanoscale liquid chromatography coupled to electrospray ionization quadrupole‐time‐of‐flight mass spectrometry, we have identified and sequenced a novel B type allatostatin (CbAST‐B1), VPNDWAHFRGSWamide, present in the pericardial organs of the crabs, Cancer borealis , and Cancer productus . We describe the physiological actions of CbAST‐B1 on the pyloric rhythm of the stomatogastric ganglion of the crab, Cancer borealis . CbAST‐B1 reduces the pyloric network frequency in a dose‐dependent manner. The effect of bath‐applied CbAST‐B1 depends on the preceding physiological state of the preparation. Surprisingly, despite marked amino‐acid sequence dissimilarity between the novel CbAST‐B1 and the A type allatostatin family of peptides (AST‐A), the physiological effects of CbAST‐B1 are similar to those of AST‐A.