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Formation of cellular projections in neural progenitor cells depends on SK3 channel activity
Author(s) -
Liebau Stefan,
Vaida Bianca,
Proepper Christian,
Grissmer Stephan,
Storch Alexander,
Boeckers Tobias M.,
Dietl Paul,
Wittekindt Oliver H.
Publication year - 2007
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2006.04437.x
Subject(s) - neuroscience , channel (broadcasting) , progenitor cell , neural activity , progenitor , neural stem cell , chemistry , biology , microbiology and biotechnology , computer science , telecommunications , stem cell
Ion channels are potent modulators for developmental processes in progenitor cells. In a screening approach for different ion channels in neural progenitor cells (NPCs) we observed a 1‐ethyl‐2‐benzimidazolinone (1‐EBIO) activated inward current, which could be blocked by scyllatoxin (ScTX, IC 50 = 2 ± 0.3 nmol/L). This initial evidence for the expression of the small conductance Ca 2+ activated K + ‐channel SK3 was confirmed by the detection of SK3 transcripts and protein in NPCs. Interestingly, SK3 proteins were highly expressed in non‐differentiated NPCs with a focused localization in lamellipodia as well as filopodial structures. The activation of SK3 channels using 1‐EBIO lead to an immediate filopodial sprouting and the translocation of the protein into these novel filopodial protrusions. Both effects could be prevented by the pre‐incubation of NPCs with ScTX. Our study gives first evidence that the formation and prolongation of filopodia in NPCs is, at least in part, effectively induced and regulated by SK3 channels.