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Tubulin cofactor B plays a role in the neuronal growth cone
Author(s) -
LopezFanarraga M.,
Carranza G.,
Bellido J.,
Kortazar D.,
Villegas J. C.,
Zabala J. C.
Publication year - 2007
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2006.04328.x
Subject(s) - growth cone , tubulin , microtubule , biology , microbiology and biotechnology , biogenesis , neurogenesis , microtubule associated protein , neurite , gene knockdown , biochemistry , axon , gene , in vitro
Tubulin cofactors, initially identified as α‐, β‐tubulin folding proteins, are now believed to participate in the complex tubulin biogenesis and degradation routes, and thus to contribute to microtubule functional diversity and dynamics. However, a concrete role of tubulin cofactor B (TBCB) remains to be elucidated because this protein is not required for tubulin biogenesis, and it is apparently not essential for life in any of the organisms studied. In agreement with these data, here we show that TBCB localizes at the transition zone of the growth cones of growing neurites during neurogenesis where it plays a role in microtubule dynamics and plasticity. Gene silencing by means of small interfering RNA segments revealed that TBCB knockdown enhances axonal growth. In contrast, excess TBCB, a feature of giant axonal neuropathy, leads to microtubule depolymerization, growth cone retraction, and axonal damage followed by neuronal degeneration. These results provide an important insight into the understanding of the controlling mechanisms of growth cone microtubule dynamics.