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Autoradiographical distribution of imidazoline binding sites in monoamine oxidase A deficient mice
Author(s) -
Anderson Neil J.,
Seif Isabelle,
Nutt David J.,
Hudson Alan L.,
Robinson Emma S. J.
Publication year - 2006
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2006.03662.x
Subject(s) - monoamine oxidase , imidazoline receptor , monoamine oxidase b , monoamine neurotransmitter , medicine , endocrinology , distribution (mathematics) , chemistry , biology , biochemistry , serotonin , enzyme , mathematics , receptor , mathematical analysis
This study has used receptor autoradiography to characterize imidazoline binding sites (I‐BS) in monoamine oxidase (MAO) A knockout and wild‐type mice. A comparison between MAO‐A and MAO‐B, binding of the endogenous β‐carboline [ 3 H]harmane, and I‐BS, has been made using sections from brain and kidney. The loss of binding to MAO‐A in the knockout animals was confirmed using the selective radioligand [ 3 H]Ro41–1049, with labelling reduced to background levels. The binding of [ 3 H]Ro19–6327 to MAO‐B was unaffected, indicating no change in this isoform in response to the loss of MAO‐A. A reduction in binding to the I 2 ‐BS, as labelled by both [ 3 H]idazoxan and [ 3 H]2‐BFI (2‐(2‐benzofuranyl)‐2‐imidazoline), was seen in the MAO‐A knockout animals in both brain and kidney sections, whereas binding to the I 1 ‐BS in kidney sections remained unchanged. The loss of I 2 binding was found to be regionally dependent and was positively correlated with the relative expression of MAO‐A in specific regions in the wild‐type animals. Using the MAO‐A knockout mice it was also possible to demonstrate a non‐MAO‐A population of binding sites labelled by the putative I‐BS endogenous ligand, harmane. 2