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Minocycline exerts inhibitory effects on multiple mitogen‐activated protein kinases and IκBα degradation in a stimulus‐specific manner in microglia
Author(s) -
Nikodemova Maria,
Duncan Ian D.,
Watters Jyoti J.
Publication year - 2006
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2005.03520.x
Subject(s) - minocycline , microglia , mapk/erk pathway , p38 mitogen activated protein kinases , kinase , microbiology and biotechnology , protein kinase a , mitogen activated protein kinase , chemistry , inhibitory postsynaptic potential , biology , pharmacology , inflammation , neuroscience , biochemistry , immunology , antibiotics
Abstract CNS inflammation mediated by microglial activation can result in neuronal and glial cell death in a variety of neurodegenerative and demyelinating diseases. Minocycline, a second‐generation tetracycline, has profound anti‐inflammatory properties in the CNS mediated, in part, by inhibition of microglia. MAPK and nuclear factor‐κB (NF‐κB) activation are hallmarks of activated microglia and they are critical for the expression of many inflammatory mediators. In the present study, we investigated minocycline effects on activation of p38, c‐Jun‐N‐terminal activated protein kinase (JNK) 1/2 and extracellular signal regulated kinase (ERK) 1/2 MAPKs and inhibitor α of NF‐κB (IκBα) degradation in BV‐2 and primary microglial cells. Our results demonstrate that minocycline has the ability to inhibit all MAPKs but these effects strongly depend on the stimulus used for MAPK activation. Minocycline significantly decreased activation of all lipopolysaccharide‐stimulated MAPKs but it was without effect on MAPKs activated by H 2 O 2 . Minocycline inhibited JNK1/2 and ERK1/2 but not p38 when stimulated by 2′,3′‐ O ‐(4‐benzoylbenzoyl)‐adenosine 5′‐triphosphate, indicating that minocycline affects only certain upstream signaling target(s) that are stimulus‐specific. Our data also suggest that protein kinase C (PKC) inhibition may be partially involved in the minocycline mechanism of MAPK inhibition. In addition, minocycline attenuated lipopolysaccharide‐stimulated degradation of IκBα implying a possible inhibitory role on NF‐κB transcriptional activity.