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A developmental profile of the levels of calcium pumps in chick cerebellum
Author(s) -
Sepúlveda M. Rosario,
HidalgoSánchez Matías,
Mata Ana M.
Publication year - 2005
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2005.03401.x
Subject(s) - serca , cerebellum , biology , microbiology and biotechnology , choroid plexus , atpase , purkinje cell , plasma membrane ca2+ atpase , endoplasmic reticulum , medicine , endocrinology , biochemistry , central nervous system , enzyme
The functional expression and distribution of intracellular ATPase (sarco(endo)plasmic reticulum Ca 2+ ‐ATPase: SERCA) and plasma membrane Ca 2+ ‐ATPase (PMCA) was analyzed in the developing chick cerebellum. The activity and Ca 2+ uptake increase with development for both ATPases. However, the protein content increases with the stage of development only for SERCA, remaining constant for PMCA. Immunohistochemical assays showed that the ontogenesis of these ATPases goes along with definite stages of cerebellum histogenesis, and is complete at hatching. The SERCA is mainly distributed in Purkinje neurons, whereas the PMCA seems to be expressed initially in climbing fibers, shifting to soma and spiny branchlets of Purkinje cells at late embryonic stages. Granule cells express both ATPases according to their degree of maturity, whereas only PMCA is present in cerebellar glomeruli. These pumps are present in deep nuclei and the choroid plexus, although in this latter tissue their expression declines with development. The spatio‐temporal distribution of SERCA and PMCA must be closely related to their association with the development of specific cells and processes of the chick cerebellum.