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Regulation of A2B adenosine receptor functioning by tumour necrosis factor a in human astroglial cells
Author(s) -
Trincavelli Maria L.,
Marroni Matteo,
Tuscano Daniela,
Ceruti Stefania,
Mazzola Alessia,
Mitro Nico,
Abbracchio Maria P.,
Martini Claudia
Publication year - 2004
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2004.02793.x
Subject(s) - agonist , medicine , adenosine , endocrinology , receptor , adenylyl cyclase , astrogliosis , adenosine a1 receptor , biology , adenosine receptor , adenosine a3 receptor , stimulation , chemistry , central nervous system
Low‐affinity A 2B adenosine receptors (A 2B ARs), which are expressed in astrocytes, are mainly activated during brain hypoxia and ischaemia, when large amounts of adenosine are released. Cytokines, which are also produced at high levels under these conditions, may regulate receptor responsiveness. In the present study, we detected A 2B AR in human astrocytoma cells (ADF) by both immunoblotting and real‐time PCR. Functional studies showed that the receptor stimulated adenylyl cyclase through Gs proteins. Moreover, A 2B ARs were phosphorylated and desensitized following stimulation of the receptors with high agonist concentration. Tumour necrosis factor alpha (TNF‐α) treatment (24‐ h) increased A 2B AR functional response and receptor G protein coupling, without any changes in receptor protein and mRNA levels. TNF‐α markedly reduced agonist‐dependent receptor phosphorylation on threonine residues and attenuated agonist‐mediated A 2B ARs desensitization. In the presence of TNF‐α, A 2B AR stimulation in vitro induced the elongation of astrocytic processes, a typical morphological hallmark of in vivo reactive astrogliosis. This event was completely prevented by the selective A 2B AR antagonist MRS 1706 and required the presence of TNF‐α. These results suggest that, in ADF cells, TNF‐α selectively modulates A 2B AR coupling to G proteins and receptor functional response, providing new insights to clarify the pathophysiological role of A 2B AR in response to brain damage.