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Identification of GABA A receptor subunit variants in midbrain dopaminergic neurons
Author(s) -
Okada Hideki,
Matsushita Natsuki,
Kobayashi Kenta,
Kobayashi Kazuto
Publication year - 2004
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2004.02271.x
Subject(s) - ventral tegmental area , substantia nigra , pars compacta , midbrain , tyrosine hydroxylase , dopaminergic , biology , dopamine , protein subunit , neuroscience , microbiology and biotechnology , biochemistry , central nervous system , gene
Modulation of the activity of dopamine (DA)‐producing neurons by GABA plays an important role in the control of DA‐mediated brain functions. Ionotropic GABA A receptors exist as heteropentametric structures assembling different subunits composed of various subtypes. However, the expression pattern of these subunits in DA neurons in the ventral midbrain has not been fully defined. In the present study, we investigated the subunit composition of GABA A receptors in DA neurons in the substantia nigra pars compacta (SNc) and the ventral tegmental area (VTA). We isolated DA neurons from the ventral midbrain of transgenic mice that express green fluorescent protein under the control of the tyrosine hydroxylase (TH) gene promoter and analyzed expression of various GABA A receptor subunits in single cells by using the reverse transcription‐polymerase chain reaction. This analysis showed the presence of the transcripts encoding α2, α3, α4, β1, β3 and γ2 subunits in the isolated DA neurons. Double fluorescence in situ hybridization with probes for TH and GABA A receptor subunit mRNAs revealed the expression of these six subunits in the majority of DA neurons in the SNc and the VTA.