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Alterations in Nitric Oxide‐Stimulated Endogenous ADP‐Ribosylation Associated with Long‐Term Potentiation in Rat Hippocampus
Author(s) -
Duman Ronald S.,
Terwilliger Rose Z.,
Nestler Eric J.
Publication year - 1993
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1993.tb13652.x
Subject(s) - long term potentiation , endogeny , hippocampus , nitric oxide , neuroscience , chemistry , medicine , endocrinology , biology , biochemistry , receptor
The present study examines the possible involvement of nitric oxide (NO)‐stimulated endogenous ADP‐ribosylation in long‐term potentiation (LTP). LTP was induced in hippocampal slices by stimulation of Schaffer collateral inputs to the CA1 pyramidal neurons. Basal and sodium nitroprusside (SNP), which generates NO, stimulation of endogenous ADP‐ribosylation was then studied in CA1 subfields isolated from the slices. Control slices received no treatment or were tetanized in the presence of aminophosphonovaleric acid, an NMDA receptor antagonist that blocks the development of LTP. SNP‐stimulated ADP‐ribosylation of endogenous proteins was reduced by 40–70% in LTP slices relative to control slices. LTP was also associated with a small but significant reduction in basal ADP‐ribosylation activity. The results demonstrate that the induction of LTP is associated with regulation of endogenous ADP‐ribosylation and suggest a role for this type of covalent modification in some aspect of LTP.