Premium
Difference in the Effectiveness of Ca 2+ to Evoke Catecholamine Secretion Between Adrenaline‐and Noradrenaline‐Containing Cells of Bovine Adrenal Medulla
Author(s) -
Teraoka Hiroki,
Sugawara Takeshi,
Nakazato Yoshikazu
Publication year - 1993
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1993.tb13423.x
Subject(s) - adrenal medulla , catecholamine , endocrinology , digitonin , medicine , secretion , chromaffin cell , acetylcholine , biology , adrenal gland , chemistry , biochemistry , enzyme
Differential adrenaline (Ad) and noradrenaline (NA) secretions evoked by secretagogues were investigated using digitonin‐permeabilized adrenal chromaffin cells, cultured adrenal chromaffin cells, and perfused adrenal glands of the ox. In digitonin‐permeabilized cells, Ca 2+ (0.8‐160 μM) caused a concentration‐dependent increase in catecholamine secretion, which was characterized by a predominance of NA over Ad secretion. Acetylcholine (10‐1,000 μM), high K + (14‐56 μM), and bradykinin (0.1‐1,000 μM) all were confirmed to induce the release of more NA than Ad at all concentrations used. There was no apparent difference in the ratios of NA/Ad between Ca 2+ ‐induced catecholamine secretion from digitonin‐permeabilized cells and those induced by secretagogues from cultured cells. Qualitatively the same result was obtained in the secretory responses to acetylcholine and high K + in perfused adrenal glands. These results indicate that the effectiveness of Ca 2+ for catecholamine secretion is higher in the secretory apparatus of NA cells than in that of Ad cells of the bovine adrenal medulla. This may be one of the reasons why the secretagogues cause a predominance of NA secretion over Ad secretion in the bovine adrenal medulla.