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Analysis of the Human Dopamine β‐Hydroxylase Promoter: Transcriptional Induction by Cyclic AMP
Author(s) -
Lamouroux Annie,
Houhou Leila,
Biguet Nicole Faucon,
SerckHanssen Guldborg,
Guibert Bernard,
IcardLiepkalns Christine,
Mallet Jacques
Publication year - 1993
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1993.tb05861.x
Subject(s) - reporter gene , microbiology and biotechnology , gene , transcriptional regulation , transcription (linguistics) , gene expression , tyrosine hydroxylase , biology , cell culture , transfection , dopamine , dopamine beta monooxygenase , endocrinology , genetics , linguistics , philosophy
We have analyzed some functional aspects of the promoter of the human dopamine β‐hydroxylase (DBH) gene. A fragment of 1,247 bp directly 5′ to the transcriptional start was progressively shortened, placed in front of a reporter gene, and tested in a human neuroblastoma cell line expressing DBH (SK‐N‐SH‐TFM) and in a monkey kidney cell line (CV‐1). A remarkably short region (267 bp), directly upstream from the transcription start, was sufficient to confer activity and tissue‐specific expression. Furthermore, the expression of the DBH gene was shown to be inducible by cyclic AMP in SK‐N‐SH‐TFM cells. This effect was demonstrated to occur at the transcriptional level, as shown by run‐on assays, and was due to the presence of a near‐consensus cyclic AMP‐responsive element located in the untranscribed 5′ regulatory region of the gene.