Premium
Chronic Ethanol Reduces Immunologically Detectable G qα/11α in NG108–15 Cells
Author(s) -
Williams Robert J.,
Kelly Eamonn
Publication year - 1993
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1993.tb03637.x
Subject(s) - ethanol , g protein , alpha (finance) , receptor , g alpha subunit , chemistry , guanine , biochemistry , microbiology and biotechnology , biology , protein subunit , nucleotide , medicine , construct validity , nursing , gene , patient satisfaction
Recent work has shown that chronic ethanol treatment inhibits receptor‐stimulated phosphoinositide hydrolysis in NG108–15 cells and that ethanol exerts this effect primarily at the level of the guanine‐nucleotide binding protein (G protein). Here we investigated the effects of ethanol exposure on the expression of G qα/11α , two highly homologous G protein α‐subunits that have been implicated as regulators of phosphoinositidase C. Addition of ethanol (10–200 m M ) to the culture medium for 48 h caused a concentration‐dependent decrease in the immunologically detectable levels of G qα/11α . A small (∼15%) reduction in G qα/11α , was observed after only 6 h of exposure to 200 m M ethanol, but membrane levels were reduced by 31% at 48 h. The ethanol‐induced loss of G qα/11α was apparently independent of factors present in the foetal calf serum component of the culture medium. These results suggest that the decrease in receptor‐mediated phosphoinositide hydrolysis following chronic ethanol treatment of NG108–15 cells may be mediated in part by a reduction in the membrane levels of G qα/11α .