Premium
Serotonin Modulates Nicotinic Responses of Adrenal Chromaffin Cells
Author(s) -
Vijayaraghavan Sukumar,
Schmid Herbert A.,
Mapp Karen S.
Publication year - 1993
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1993.tb03571.x
Subject(s) - chromaffin cell , nicotine , catecholamine , endocrinology , medicine , nicotinic agonist , serotonin , receptor , adrenal medulla , chemistry , acetylcholine , desensitization (medicine) , acetylcholine receptor , biology
5‐Hydroxytryptamine (5‐HT) specifically and reversibly inhibits nicotine‐induced currents and catecholamine release in bovine adrenal chromaffin cells in culture. Pharmacological analysis indicates that the inhibition is not mediated by known 5‐HT receptor subtypes. The inhibition is noncompetitive over a range of nicotine concentrations between 1 and 100 μM. Preincubation with either 5‐HT or substance P significantly protects the response from nicotine‐induced desensitization. It is concluded that 5‐HT inhibits nicotinic acetylcholine receptors on bovine adrenal chromaffin cells, probably by binding to a noncompetitive site on the receptor itself. Because both blood and the chromaffin cells contain 5‐HT, the inhibition provides an opportunity for negative control of catecholamine secretion from the adrenals.