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Identification of Tryptophan 2,3‐Dioxygenase RNA in Rodent Brain
Author(s) -
Haber Roberta,
Bessette Daniel,
HulihanGiblin Bridget,
Durcan Michael J.,
Goldman David
Publication year - 1993
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1993.tb03269.x
Subject(s) - rodent , identification (biology) , rodent model , tryptophan , rna , dioxygenase , biology , computational biology , biochemistry , gene , ecology , amino acid , endocrinology
The gene for tryptophan 2,3‐dioxygenase (TDO) heretofore was believed to be expressed only in liver. The data presented here demonstrate that RNA encoding TDO is present in rodent brain. Oligonucleotide primers based on the rat liver TDO cDNA sequence were synthesized and used to amplify RNA derived from mouse whole brain and liver and rat brain regions by the RNA‐PCR. Reaction products were purified and subjected to DNA sequencing. Identical sequences were obtained when mouse whole brain and liver RNAs were amplified, and these sequences were shown to be 96% identical to the published rat liver tryptophan TDO cDNA sequence. In addition, TDO sequences were found in RNA derived from rat brainstem, cerebellum, cortex, hypothalamus, and the remainder of the brain.