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Dopamine D 1 Agonist SKF 38393 Increases the State of Phosphorylation of ARPP‐21 in Substantia Nigra
Author(s) -
Tsou Kang,
Girault JeanAntoine,
Greengard Paul
Publication year - 1993
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1993.tb03252.x
Subject(s) - substantia nigra , agonist , dopamine , neuroscience , chemistry , dopamine receptor d1 , phosphorylation , midbrain , dopamine receptor , endocrinology , pharmacology , medicine , biology , dopaminergic , receptor , central nervous system , biochemistry
ARPP‐21 is a cyclic AMP‐regulated phosphoprotein (M r = 21,000) that has a distribution in brain similar to that of DARPP‐32 (dopamine‐ and cyclic AMP‐regulated phosphoprotein, M r = 32,000). It is enriched in the medium‐sized spiny neurons in the striatum and in the striatonigral nerve terminals in the pars reticulata of the substantia nigra. The present study shows that dopamine D 1 agonist SKF 38393 increases the state of phosphorylation of ARPP‐21 by 26% in nigral slices and that pretreatment of the slices with D 1 antagonist SCH 23390 blocks this effect. These results demonstrate that ARPP‐21 is a dopamine‐regulated phosphoprotein. Because D 1 receptors are localized on nerve terminals of striatonigral pathway, the phosphorylation of ARPP‐21 is likely to mediate some of the intracellular effects of dopamine on these terminals.