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Calcium Regulation of Vasoactive Intestinal Polypeptide mRNA Abundance in SH‐SY5Y Human Neuroblastoma Cells
Author(s) -
Adler E. M.,
Fink J. Stephen
Publication year - 1993
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1993.tb02179.x
Subject(s) - vasoactive intestinal peptide , forskolin , junb , messenger rna , second messenger system , endocrinology , medicine , adenylate kinase , biology , calcium , signal transduction , chemistry , gene expression , microbiology and biotechnology , receptor , biochemistry , neuropeptide , gene , stimulation
Second messenger regulation of gene expression provides a mechanism by which neurons can transduce environmental stimuli into long‐term changes in the expression of molecules involved in neuronal signaling. We have investigated calcium‐dependent induction of vasoactive intestinal polypeptide (VIP) mRNA and compared it with induction of VIP mRNA by cyclic AMP. Depolarization with 60 mM KCI or exposure to the calcium ionophore A23187 increases VIP mRNA levels in SH‐SY5Y cells. The increase in VIP mRNA content in response to Ca 2+ mobilization is slow, independent of adenylate cyclase activation, and requires de novo protein synthesis. The increase in VIP mRNA content in response to elevation of cyclic AMP levels by forskolin/isobutylmethylxanthine is independent of Ca 2+ influx and does not require new protein synthesis. The mRNA for the transcription factors ATF‐3, c‐fos, c‐jun, junB , and zif/268 is induced by A23187. Of these, ATF‐3 showed the greatest relative induction by A23187 compared with induction by forskolin/isobutyl‐methylxanthine.