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Identification of Plasmolipin as a Major Constituent of White Matter Clathrin‐Coated Vesicles
Author(s) -
Sapirstein Victor S.,
Nolan Charles E.,
Stern Richard,
GrayBoard Gladys,
Beard Margaret E.
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb11352.x
Subject(s) - vesicle , myelin , white matter , oligodendrocyte , synaptic vesicle , biochemistry , chemistry , biology , biophysics , membrane , microbiology and biotechnology , central nervous system , neuroscience , medicine , radiology , magnetic resonance imaging
We have isolated and characterized coated vesicles from bovine white matter and compared them to those isolated from gray matter. The virtual absence of synaptic vesicle antigens in the white matter coated vesicles indicates they are distinct from those found in gray matter and from vesicles derived from synaptic membranes. The white matter coated vesicles also lack compact myelin components, e.g., the myelin proteolipid, galactocerebroside, and sulfatides, as well as the periaxolemmal myelin marker myelin‐associated glycoprotein. On the other hand, these vesicles contain 2′,3′‐cyclic nucleotide phosphohydrolase. The vesicles also contain high levels of plasmolipin, a protein present in myelin and oligodendrocytes. Plasmolipin was found to be four to five times higher in white matter coated vesicles than in gray matter coated vesicles. Based on western blot quantitation, the concentration of plasmolipin in white matter coated vesicles is 3–4% of the vesicle bilayer protein. These studies indicate that a significant proportion of coated vesicles from white matter may be derived from unique membrane domains of the myelin complex or oligodendroglial membrane, which are enriched in plasmolipin.