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Endopeptidase 24‐16 in Murines: Tissue Distribution, Cerebral Regionalization, and Ontogeny
Author(s) -
Dauch P.,
Masuo Y.,
Vincent J. P.,
Checler F.
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb11021.x
Subject(s) - globus pallidus , biology , striatum , endocrinology , cerebral cortex , olfactory bulb , medicine , cerebellum , hypothalamus , ontogeny , endopeptidase , hippocampal formation , cerebrum , neuropeptide , central nervous system , basal ganglia , receptor , biochemistry , dopamine , enzyme
The tissue distribution, cerebral regionalization, and ontogeny of endopeptidase 24–16 were established in murines by means of its quenched fluorimetric substrate, Mcc‐Pro‐Leu‐Gly‐Pro‐D‐Lys‐Dnp, and its selective dipep‐tide blocker, Prolie. Endopeptidase 24–16 was particularly abundant in the liver and kidney, and the lowest specific activity was detected in the heart. In the brain, a 16‐fold difference in specific activity was observed between the poorest and the richest cerebral areas. Endopeptidase 24–16 appeared in high concentrations in the olfactory bulb and tubercule, cingulate cortex, medial striatum, and globus pallidus, and was particularly weak in the CA1, CA2, and CA3 parts of the hippocampal formation and in the cerebellum. Endopeptidase 24–16 content in thirteen thalamic nuclei indicated a rather homogeneous distribution. This homogeneity was not observed in the hypothalamus, where pronounced variations occurred between enriched zones such as suprachiasmatic and arcuate nuclei and relatively poor areas such as periventricular and supraoptic nuclei. Endopeptidase 24–16 appeared to be developmentally regulated in the mouse brain; it was already detected at the fetal stage, increased transiently after birth, then regularly declined until adulthood.