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Changes in Aldose Reductase After Crush Injury of Normal Rat Sciatic Nerve
Author(s) -
Wong Elsie,
Mizisin Andrew P.,
Garrett Robert S.,
Miller Arnold L.,
Powell Henry C.
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb10966.x
Subject(s) - schwann cell , wallerian degeneration , immunogold labelling , sciatic nerve , aldose reductase , axon , crush injury , chemistry , regeneration (biology) , biology , pathology , anatomy , microbiology and biotechnology , enzyme , ultrastructure , biochemistry , medicine , surgery
The response of aldose reductase (AR) to crush injury was studied in normal rat sciatic nerve. Enzyme activity and immunoreactivity of AR were determined at intervals of 1, 5, 14, 28, and 35 days after crush and correlated with histologic and immunocytochemical observations. During nerve degeneration in the distal segments of crushed nerves, a significant reduction in AR activity was detected. At 5 and 14 days, coincident with Schwann cell proliferation, enzyme activity decreased by nearly two‐ and fourfold, respectively. Although activity of AR increased by 28 days during nerve regeneration, it was not restored to normal levels at 35 days. Similar reductions were observed with the immunoblotting of the enzyme. Quantitative analysis of immunogold labelling on electron micrographs confirmed that proliferating as well as remyelinating Schwann cells contained reduced gold particle density compared to Schwann cells of noncrushed myelinated fibers. Immunoblots of P 0 , a marker for the degree of Schwann cell differentiation or myelination, showed that the temporal sequence of changes in P 0 paralleled that of AR. Thus expression of AR is a function of differentiated or mature Schwann cells. The putative volume regulatory role of AR in Schwann cells may become superfluous during Wallerian degeneration.