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Stimulus‐Induced Accumulation of Inositol Tetrakis‐, Pentakis‐, and Hexakisphosphates in N1E‐115 Neuroblastoma Cells
Author(s) -
Sasakawa Nobuyuki,
Nakaki Toshio,
Kashima Reiko,
Kanba Shigenobu,
Kato Ryuichi
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb10953.x
Subject(s) - inositol , chemistry , endocrinology , inositol phosphate , medicine , inositol trisphosphate , stimulation , potency , stereochemistry , receptor , biochemistry , biology , in vitro
When [ 3 H]inositol‐prelabelled N1E‐115 cells were stimulated with carbamylcholine (CCh) (100 μ M ), high K + (60 mM), and prostaglandin E, (PGE,) (10 μ M ), a transient increase in [ 3 H]inositol pentakisphosphate (InsP 5 ) accumulation was observed. The accumulation reached its maximum level at 15 s and had declined to the basal level at 2 min. CCh, high K + , and PGE, also caused accumulations of [ 3 H]inositol 1,4,5‐trisphosphate [Ins(1,4,5)P 3 ], [ 3 H]inositol 1,3,4,6‐tetrakisphosphate [Ins(1,3,4,6)P 4 ], and 1 3 H]inositol hexakisphosphate (InsP 6 ). Muscarine and CCh induced accumulations of [ 3 H]Ins(1,4,5)P 3 , [ 3 H]‐Ins(1,3,4,6)P 4 , [ 3 H]InsP 5 , and [ 3 H]InsP 6 with a similar potency and exerted these maximal effects at 100 μ M , whereas nicotine failed to do so at 1 m M. With a slower time course, CCh, high K + , and PGE 1 caused accumulations of [ 3 H]‐inositol 1,3,4‐trisphosphate [Ins(1,3,4)P 3 ] and [ 3 H]inositol 1,3,4,5‐tetrakisphosphate [Ins(1,3,4,5)P 4 ]. In an N1E‐115 cell homogenate, [ 3 H]Ins(1,4,5)P 3 , [ 3 H]Ins(1,3,4,5)P 4 , and [ 3 H]Ins(1,3,4)P 3 were converted to [ 3 H]InsP 5 through [ 3 H]‐Ins(1,3,4,6)P 4 . The above results indicate that Ins(1,3,4,6)P 4 , InsP 5 , and InsP 6 are rapidly formed by several kinds of stimulants in N1E‐115 cells.