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Synthesis of Glial Fibrillary Acidic Protein in Rat C 6 Glioma in Chemically Defined Medium: Cyclic AMP‐Dependent Transcriptional and Translational Regulation
Author(s) -
Messens Joris,
Slegers Herman
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb10948.x
Subject(s) - glial fibrillary acidic protein , messenger rna , intracellular , protein biosynthesis , stimulation , vimentin , translation (biology) , lipopolysaccharide , biology , astrocyte , microbiology and biotechnology , chemistry , biochemistry , endocrinology , gene , central nervous system , immunology , immunohistochemistry
Glial fibrillary acidic protein (GFA) expression was induced in rat C 6 glioma in chemically defined medium by the addition of N 6 , O 2′ ‐dibutyryl cyclic AMP (dbcAMP). Induction was dependent on the increase in intracellular cyclic AMP (cAMP), which was linearly correlated with added dbcAMP. Contrary to GFA mRNA synthesis, which can be obtained by cAMP‐dependent and ‐independent pathways, translation of mRNA into GFA was observed only above a cellular cAMP concentration of approximately 0.2 fmol/cell. dbcAMP stimulation did not affect the vimentin concentration, which remained at a low level, but changed the cellular morphology from a bipolar to a stellate shape. A similar morphological change was observed after stimulation of C 6 with lipopolysaccharide (LPS). However, LPS did not significantly increase the intracellular concentration of cAMP and the LPS‐induced mRNA was not translated into GFA. Our results indicate that GFA synthesis is regulated at the mRNA level and at the translational level and that a cAMP‐dependent mechanism determines the ultimate synthesis of GFA by a yet unknown mechanism.