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Fetal‐Type Phosphorylation of the τ in Paired Helical Filaments
Author(s) -
Kanemaru Kazutomi,
Takio Koji,
Miura Reiko,
Titani Koiti,
Ihara Yasuo
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb10039.x
Subject(s) - antiserum , phosphorylation , chemistry , fetus , kinase , microbiology and biotechnology , antibody , biochemistry , biology , immunology , genetics , pregnancy
Abstract: To determine the phosphorylation sites of the τ in paired helical filaments (PHF), two types of PHF antisera with different specificities were used: One was a conventional anti‐PHF, and the other was an antiserum to formic acid‐denatured PHF (anti‐HFoPHF). Phosphorylated τ‐specific antibodies, anti‐ptau 1 and anti‐ptau 2, were prepared from anti‐PHF and anti‐HFoPHF, respectively. We found that both anti‐ptau 1 and anti‐ptau 2 labeled fetal or juvenile τ but not adult τ. The anti‐ptau 1‐ and anti‐ptau 2‐recognition sites were immunochemically localized to the fragment Asp 313 to Ile 328 in the most COOH‐terminal portion of τ. Furthermore, Ser 315 was determined as the anti‐ptau 2 recognition site. The sequence surrounding Ser 315 was not found in the canonical sequences phosphorylated with known kinases.