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Synapsin I Regulates Glutamate Release from Rat Brain Synaptosomes
Author(s) -
Nichols Robert A.,
Chilcote Tamie J.,
Czernik Andrew J.,
Greengard Paul
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09788.x
Subject(s) - synapsin i , synapsin , glutamate receptor , neurotransmitter , synaptosome , phosphorylation , protein kinase a , biology , chemistry , biochemistry , microbiology and biotechnology , central nervous system , neuroscience , synaptic vesicle , receptor , membrane , vesicle
Introduction of the dephosphorylated form of synapsin 1 into rat brain synaptosomes using freeze‐thaw (transient) perme‐abilization significantly decreased the K + ‐induced release of glutamate. In contrast, introduction of synapsin I that had been phosphorylated by Ca 2+ /calmodulin‐dependent protein kinase II was without effect on glutamate release. Addition of dephosphosynapsin I after freeze‐thaw treatment also had no effect. Thus, the action of synapsin I was dependent on the phosphorylation state of synapsin I and on its entry into the synaptosomes. Our results implicate synapsin I as an important component in the regulation of neurotransmitter release in the mammalian nervous system.