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Affinity Purification of Human τ Proteins and the Construction of a Sensitive Sandwich Enzyme‐Linked Immunosorbent Assay for Human τ Detection
Author(s) -
Mercken Marc,
Vandermeeren Mark,
Lübke Ursula,
Six Jan,
Boons Jef,
Vanmechelen Eugène,
Voorde Andre,
Gheuens Jan
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09754.x
Subject(s) - polyclonal antibodies , monoclonal antibody , affinity chromatography , enzyme , microbiology and biotechnology , antibody , chemistry , monoclonal , chromatography , human brain , biochemistry , biology , immunology , neuroscience
Immunoaffinity chromatography with a monoclonal antibody produced against bovine τ protein was used to purify τ proteins from human brain. Fifty grams of brain tissue yielded τ 2 mg of pure τ proteins. The affinity‐purified human τ was used to produce a high‐titered rabbit anti‐human τ serum. The monoclonal anti‐τ antibody and the polyclonal rabbit anti‐τ serum were then used to construct a sandwich enzyme‐linked immunosorbent assay for detection of human τ proteins, with a sensitivity of 1 ng/ml.