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Analysis of Muscarinic Receptor Subtypes in the Mouse Cochlea by Means of the Polymerase Chain Reaction
Author(s) -
Drescher Dennis G.,
Upadhyay Sanjiv,
Wilcox Edward,
Fex Jörgen
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09436.x
Subject(s) - muscarinic acetylcholine receptor , microbiology and biotechnology , complementary dna , biology , reverse transcription polymerase chain reaction , messenger rna , receptor , cochlea , reverse transcriptase , polymerase chain reaction , rna , genomic dna , dna , chemistry , biochemistry , gene , anatomy
Total RNA was extracted with guanidine thiocyanate from the cochleas of 16‐day‐old CBA J mice. The mRNA was purified from the total RNA using oligo‐dT cellulose, and the mRNA was treated with DNase to degrade genomic DNA. After reverse transcription, resulting cDNA was amplified by polymerase chain reaction (PCR), using primers specific for the nucleotide sequences m 1 ‐m 5 , representing subtypes of muscarinic acetylcholine receptors. PCR products corresponding to subtypes m 1 , m 3 , and m 5 , but not to m 2 and m 4 , were amplified. These results suggest that muscarinic acetylcholine receptors of these odd‐numbered subtypes are expressed in the mammalian cochlea.