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Activity‐Dependent Regulation of Na + ,K + ‐ATPase α Isoform mRNA Expression In Vivo
Author(s) -
Mata Marina,
Hieber Virginia,
Beaty Michael,
Clevenger Michael,
Fink David J.
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09415.x
Subject(s) - gene isoform , messenger rna , in situ hybridization , hypothalamus , microbiology and biotechnology , in vivo , depolarization , biology , supraoptic nucleus , medicine , endocrinology , chemistry , biochemistry , gene
To investigate the functional role of the different Na + ,K + ‐ATPase a (catalytic) subunit isoforms in neuronal cells, we used quantitative in situ hybridization with riboprobes specific for αl, α2, and α3 isoforms to measure the level of a isoform‐specific expression in the neuroendocrine cells of the supraoptic (SON) and paraventricular (PVN) nuclei of rat hypothalamus. A prolonged increase in electrical activity of these cells, achieved by 5 days of salt treatment, increased the amount of α isoform mRNA in the SON and PVN by 50%. Levels of α mRNA in other brain regions and levels of α2 and α3 mRNAs were not affected by salt treatment. We conclude that the α1 isoform Na + ,K + ‐ATPase may be specifically adapted to pump out Na + , which enters the cells through voltage‐gated channels during neuronal depolarization.