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γ‐Glutamylaminotransferase and Transglutaminase in Subcellular Fractions of Rat Cortex and in Cultured Astrocytes
Author(s) -
Reichelt Karl L.,
Poulsen Eystein
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09398.x
Subject(s) - tissue transglutaminase , enzyme , synaptosome , centrifugation , in vitro , biochemistry , subcellular localization , cortex (anatomy) , cell fractionation , differential centrifugation , cerebral cortex , biology , chemistry , neuroglia , microbiology and biotechnology , central nervous system , neuroscience , cytoplasm
The subcellular distribution of γ‐glutamylaminotransferase (EC 2.3.2.2) and transglutaminase (EC 2.3.2.1) has been investigated in rat brain tissue fractionated by a centrifugation and sedimentation technique. Neither of these enzymes was enriched in the synaptosomal fraction. Comparing the in vitro grown astrocytes with synaptosomes, we find that both of these enzymes may possibly be more important in the glial element of the synaptic region. γ‐Glutamylaminotransferase is most abundant in capillaries, confirming previous reports.