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Enhancement of Endogenous Release of Glutamate and γ‐Aminobutyric Acid from Hippocampus CA1 Slices After In Vivo Long‐Term Potentiation
Author(s) -
Ghijsen Wim E. J. M.,
Besselsen Elly,
Geukers Vincent,
Kamphuis Willem,
Silva Fernando H. Lopes
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09395.x
Subject(s) - long term potentiation , endogeny , hippocampus , glutamate receptor , in vivo , neuroscience , aminobutyric acid , chemistry , biology , biochemistry , receptor , microbiology and biotechnology
The effect of long‐term potentiation (LTP) on endogenous amino acid release from rat hippocampus slices was studied. LTP was induced in vivo by application of a tetanus (200 Hz, 200 ms) to the Schaffer collateral fibers in unanesthetized rats. Endogenous release of glutamate and γ‐aminobutyric acid (GABA) was investigated 60 min after tetanization in CA1 subslices of potentiated and control rats. No significant effects of LTP were observed in basal and K + ‐induced Ca 2+ ‐independent release components of these amino acids. In contrast, K + ‐induced Ca 2+ ‐dependent release of both glutamate and GABA increased ∼ 100% in slices from potentiated rats. No differences were observed in total content of glutamate and GABA between the subslices from control and LTP animals. These results suggest a persistent increase in the recruitment of the presynaptic vesicular pool of glutamate and GABA during LTP.