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Preincubation with Substance P Induces Substance P‐Stimulated Phosphatidylinositol Turnover in Cultured Cerebellar Astrocytes
Author(s) -
Marriott Derek R.,
Wilkin Graham P.
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09390.x
Subject(s) - phosphatidylinositol , pi , substance p , medicine , endocrinology , receptor , cerebellum , astrocyte , second messenger system , neuroglia , chemistry , biology , microbiology and biotechnology , signal transduction , biochemistry , central nervous system , neuropeptide
In this study we have investigated the effects of preincubation of cultured astrocytes with substance P (SP) on subsequent 125 I‐Bolton—Hunter conjugated SP ( 125 I‐BHSP) receptor binding, and SP‐stimulated phosphatidylinositol (PI) accumulation. Spinal cord astrocytes preincubated for up to 96 h with SP (0.001‐1,‐n M ) suffered a dose‐dependent decrease in both subsequent 125 I‐BHSP and SP‐stimulated PI turnover. In contrast, preincubation of cerebellar astrocytes with SP resulted in an increase in SP‐stimulated PI turnover, with no change in 125 I‐BHSP receptor binding. SP‐induced PI turnover in cerebellar astrocytes was maximal after 72 h of preincubation with 0.1 n M SP. These data suggest that increased coupling between receptor and second messenger occurs in response to chronic exposure to SP.