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Increased Superoxide Dismutase Activity in Aged Human Cerebrospinal Fluid and Rat Brain Determined by Electron Spin Resonance Spectrometry Using the Spin Trap Method
Author(s) -
Hiramatsu Midori,
Kohno Masahiro,
Edamatsu Rei,
Mitsuta Keiichi,
Mori Akitane
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09375.x
Subject(s) - superoxide dismutase , cerebrospinal fluid , chemistry , cytosol , electron paramagnetic resonance , nuclear magnetic resonance , enzyme , endocrinology , medicine , biochemistry , biology , physics
Superoxide dismutase (SOD) activity in CSF of patients was determined by electron spin resonance spec‐trometry using the spin trap method. Variation in SOD activity was found among patients. SOD activity in CSF of subjects increased with age and this was identified as Cu,Zn‐SOD activity by electrophoresis. In addition, animal experiments showed that SOD activities were higher in mitochon‐drial and cytosol fractions of aged rats than in those of adult rats. This finding on aged rat brain validates the increase of SOD activity in aged human CSF.