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Effects of Forskolin, Dibutyryl Cyclic AMP, and 5′ N ‐Ethylcarboxamide Adenosine on 22 Na Uptake by Rat Brain Synaptosomes Stimulated by Veratridine
Author(s) -
Lobo M. G. B.,
Ribeiro J. A.
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09358.x
Subject(s) - veratridine , forskolin , adenosine , medicine , endocrinology , chemistry , sodium , synaptosome , sodium channel , biology , stimulation , central nervous system , organic chemistry
The effects of forskolin, dibutyryl cyclic AMP, and 5′‐ N ‐ethylcarboxamide adenosine on specific 22 Na uptake by synaptosomes stimulated by veratridine were investigated. All substances inhibited 22 Na uptake, with forskolin more potent than 5′‐ N ‐ethylcarboxamide and this latter one more potent than dibutyryl cyclic AMP. In the absence of prein‐cubation with forskolin, this substance caused little or no effect on 22 Na uptake by synaptosomes. In the presence of the adenosine antagonist dipropylsulfophenylxanthine, the inhibitory effect of 5′‐ N ‐ethylcarboxamide adenosine on 22 Na uptake was consistently antagonized. The results were interpreted as forskolin and 5′‐ N ‐ethylcarboxamide adenosine increasing cyclic AMP accumulation, and dibutyryl cyclic AMP mimicking it, and by these mechanisms decreasing sodium uptake through the sodium channels.