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Tau 2: A Probe for a Ser Conformation in the Amino Terminus of τ
Author(s) -
Watanabe Nobuo,
Takio Koji,
Hasegawa Masato,
Aral Takao,
Titani Koiti,
Ihara Yasuo
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09349.x
Subject(s) - chemistry , stereochemistry , computational biology , neuroscience , biology
We have determined the epitope for Tau 2, a monoclonal antibody that intensely stained tangles, plaque neurites, and curly fibers in the tissue section, and strongly labeled bovine τ, but only very weakly labeled human r on the blot. The epitope has been localized to Ala 95 through Ala 108 of bovine τ. Ser 101 is critical for Tau 2 reactivities; the replacement of Ser by Pro, which is found in rat, mouse, and human T, brings about very weak Tau 2 reactivities. The strong Tau 2 staining of tangles and its effective absorption with a synthetic Ser peptide (Ala 95 through Ala 108 ) suggest that the τ in paired helical filaments takes a Ser conformation, rather than a Pro conformation, in its ammo‐terminal portion.